4/6/2024 0 Comments Ape sequence viewerIn some cases where the sequences differ by a single base-pair indel, the alignment output by ApE is incorrect and the mismatching residues are not highlighted in red as expected. There is a serious bug affecting the alignment function in recent versions of ApE (I have encountered it in 2.0.39 - 2.0.45 at least, may affect all 2.0.X versions?) released over the last ~12 months, including the current release (2.0.45). The author is very responsive and integrated a BankIt export option, I suggested, within a few days. BLAST selected sequence at NCBI or wormbase.shows translation, Tm, %GC, ORF of selected DNA.restriction sites and region markup in sequence and in a simple cartoon.for primers, probes, or restriction sites pre-existing expandable feature tables which can be used for automatic annotation.export graphic files of sequence diagrams.exports BankIt feature table for sequence submission to NCBI GenBank.saves data in a modified Genbank file format (DNA Strider-compatible).imports DNA Strider, Fasta, Genbank, EMBL and ABI sequence traces.
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